BACKGROUND: Human herpesviruses may cause infections of the central nervous system (CNS). Primary infection by any of the eight viruses, usually occurring in childhood, is either asymptomatic or produces fever and rash of skin or mucousmembranes; other organs might be involved on rare occasions. Children, adults, and the elderly can all be affected. HHVs DNA was detected in 52 of 155 (33.5%) analyzed samples. This work describes, for the first time, the involvement of TLRs 3 and 7-9 in the recognition of BoHV in the bovine nervous system, indicating that the expression of these receptors might be associated with the development of neurological disease. Herpes encephalitis, an uncommon yet severe disease, represents an aberrant interaction of the virus and host that is at present poorly understood. Good methodological standards exist but few studies have adhered to these standards.
In aim 2, I will study in vivo viral invasion and spread of infection from the peripheral nervous system (the submandibular ganglia) to the central nervous system in living animals, tissue explants, and fixed tissues. Experiments are divided among three aims all featuring light and video microscopy: Imaging individual virion egress events using multi-color TIRF microscopy;assaying axon-cell egress and spread events with chambered neurons, three color virus technology, and fast epifluorescence imaging;and imaging in vivo/ex vivo PRV invasion of the PNS at the single cell and single particle level.